Researcher interests: Elucidation of the importance of different structural domains of the Na+-Ca2+ exchanger (NCX) in its functional expression. About 15 years ago our laboratory has cloned three isoforms of the rat NCX1 gene (1,2). By introducing defined changes in NCX protein structure using site directed mutagenesis, we are examining the impact of these changes on NCX function and protein expression. This approach led to elucidation of the role of different NCX domains (N-terminus, C-terminus and the large cytoplasmic loop) in maturation, trafficking to the cell surface and acquisition of function competence (3, 5,6). The role of disulfide bridges (6) and importance of individual cysteine residues is studies by SCAM and modification with membrane permeable and non permeable covalent probes. NCX expression, CsA and [Ca2+] regulation: Exposure of cells expressing NCX to Cyclosporin A (CsA)- an immunosupressive agent given to patients after organ transplantation, led to down regulation of NCX1, NCX2 and NCX3 surface expression (7,8) in a post translational manner. Cyclophilins, the family of cellular receptors for CsA play a role in protein folding (chaperon action) and acquisition of function competent conformation via peptide X-Pro bond cis-trans isomerization. The ongoing research involves: elucidation of the mode of action of CsA on NCX expression, identification of the structurally important proline residues of the protein for determination of its conformation, studying the effect of CsA on the expression pattern NCX in cell lines expressing the protein endogenously (heart, smooth and skeletal muscle, nerve and glia) and examining the connection between CsA treatment, NCX surface expression and intracellular [Ca2+] regulati
FACULTY / SCHOOL: School of Medicine - IMRIC
Contact Business Development: Shani Bullock

Selected Publications

Benayahu Elbaz, Daniela Elmaz, Mahdi Khatib, marina eskinschwartz, Michael Valitsky, Hannah Rahamimoff
immunosuppressive drugs immunophilins and functional expression of ncx isoforms (2013)| Advances in Experimental Medicine and Biology| Read more
Benayahu Elbaz, geula davidov, Michael Valitsky, Hannah Rahamimoff
cyclophilin a is involved in functional expression of the na ca2 exchanger ncx1 (2010)| Biochemistry| Read more
Ariella Alperovitch, Benayahu Elbaz, chava kimchisarfaty, michael gottesman, Hannah Rahamimoff
modulation of na ca2 exchanger expression by immunosuppressive drugs is isoform specific (2008)| Molecular Pharmacology| Read more
alperovich, elbaz, eskinshwartz, gottesman, judith kasir, kimchisarfaty, lichtenstein, Hannah Rahamimoff
cyclosporin a dependent downregulation of the na ca2 exchanger expression (2007)| Annals of the New York Academy of Sciences| Read more
judith kasir, Hannah Rahamimoff, xiaoyan ren
cysteine residues are important for functional expression of the na ca2 exchanger ncx1 in hek 293 cells (2006)| Annals of the New York Academy of Sciences| Read more
judith kasir, Hannah Rahamimoff, xiaoyan ren
the transport activity of the na ca2 exchanger ncx1 expressed in hek 293 cells is sensitive to covalent modification of intracellular cysteine residues by sulfhydryl reagents (2001)| Journal of Biological Chemistry| Read more
ian furman, judith kasir, Hannah Rahamimoff, xiaoyan ren
truncation of the c terminus of the rat brain na ca2 exchanger rbe 1 ncx1 4 impairs surface expression of the protein (1999)| Journal of Biological Chemistry| Read more
orna cook, Hannah Rahamimoff, walter low
membrane topology of the rat brain na ca2 exchanger (1998)| Biochimica et Biophysica Acta| Read more
ian furman, judith kasir, orna cook, rafi vatashski, Hannah Rahamimoff, walter low
the structural basis of na ca2 exchange activitya (1996)| Annals of the New York Academy of Sciences| Read more

Contact for more information:

Shani Bullock
VP, Business Development, Healthcare
+972-2-6586608
Contact ME: