Biologic and Genetic Marking - Memogene
Category |
Agriculture, Biotechnology |
Keywords |
Gene Editing, Plants , Viral, Virus, Delivery System, Platform CRISPR/Cas compatible guide RNAs, Zinc finger nucleases, Mega nucleases |
Current development stage |
General list: TRL9 Actual system proven in operational environment
|
Collaboration Opportunity |
Licensing of Technology Research Services
|
Background
Today's traditional plant breeding techniques (plant cross breeding, mutagenesis and genetic engineering) are labor-intensive, expensive and time-consuming. A high throughput system for genome editing would enable fast screening of a plethora of targets, with reduced time and labor costs. MemoGene™ is a novel high throughput genome breeding technology which bypasses traditional biotechnological barriers.
Our Innovation
Memogene is a proprietary high throughput genome editing delivery system for CRISPR/Cas compatible guide RNAs, or molecular nucleases (Zinc finger nucleases, Mega nucleases). MemoGene™ enables the production of tailor-made plant varieties with new traits, while also allowing for the deletion of negative and unwanted characteristics, thus increasing the value of agricultural and horticultural products.
-
High throughput gene mutagenesis
-
High throughput gene/trait validation
-
Proven in multiple crops and is not variety/species/genus-specific
-
Transient, in planta – no regeneration, non GMO developed in several crops
-
Efficient expression in planta and in protoplasts
Technology
The MemoGene takes advantage of a novel Tobacco rattle virus (TRV)-based expression system, which is capable of systemically infecting its host, either protoplasts or spreading into a variety of plant tissues via plants cells. The viral carrier system carries nucleases as its payload and is used here for gene editing - creating permanent, inheritable and site-specific gene modification. The viral carrier system can also be used for highly efficient and rapid gene/trait validation.
Opportunity
-
This technology can be licensed to interested parties in a varieties of fields
-
The researcher can also offer Research Services to interested parties
Patent
PLANT VIRAL EXPRESSION VECTORS AND USE OF SAME FOR GENERATING GENOTYPIC VARIATIONS IN PLANT GENOMES - WO2009/130695
GENERATING GENOTYPIC VARIATIONS IN PLANT GENOMES BY GAMETE INFECTION - WO2011/048600
Scientific Articles
Transient Expression of Virally Delivered Meganuclease In Planta Generates Inherited Genomic Deletions et al 2015 https://www.cell.com/molecular-plant/supplemental/S1674-2052(15)00198-7
Genome modifications in plant cells by custom-made restriction enzymes et al 2012
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1467-7652.2011.00672.x
Nontransgenic Genome Modification in Plant Cells et al 2010 http://www.plantphysiol.org/content/154/3/1079.long
Patent Status
Granted US 8,791,324; US 9,476,060; US 10,883,111; Europe 2268129