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Directional Cryopreservation of Cells Adhered to a Substrate for Cell-Culture Experiments

Braslavsky Ido, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Biochemistry, Food Science and Nutrition
YASHUNSKY Victor, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences
Bahari Liat, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Biochemistry, Food Science and Nutrition
BEIN Amir, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Biochemistry, Food Science and Nutrition

 

Thaw and go“ cell culture chip - Revolutionizing cells’ cryopreservation for fast and simple biomedical research experiments 

 

One of the main obstacles for successful cryopreservation of cells is the irreversible damage to cells organelles and membranes caused by uncontrolled growth of ice in super-cooled liquid. This has crucial impact on organ, embryo, tissue, and blood storage and transportation in medicine.

Key features

  • Pre differentiated for of required specification (differentiated, genetically modified) enabling to start the experiment immediately after thawing the cells
  • Frozen cells culture specimens in their natural morphology, intact cell monolayer, attached to a substrate and ready for use after thawing.

Advantages

  • Reducing the mechanical damage caused to the cells during the freezing process
  • Standardization of cell specimens (e.g cells passage, confluency, gene expression etc)
  • Quality control of specimens
  • No liquid nitrogen required; Preserved specimens could be stored at -80°C for a few months
  • Less toxic cryoprotectants required (DMSO etc)

 

The group has designed and fabricated a directional freezing system prototype which comprises a motorized moving cooling stage and a complimentary software for the computerized controlling of the freezing process. 

Contact for more information:

Ariela Markel
VP, Business Development, Healthcare
+972-2-6586608