2059

Antibody Microarray for Histone Modifications

Meshorer Eran, HUJI, Faculty of Science, The Alexander Silberman Institute for Life Sciences

The Need

  • Histone modifications portray the epigenetic landscape of the genome.
  • Currently, a western blot is required to determine the state of each histone modification which is extremely time and resource consuming

Key Features

  • Significant improvement in signal to noise ration due to a unique protocol 
  • The Antibody Microarray will monitor the levels of all histone modifications simultaneously. Results are expected within 2-3 hours.
  • The method is useful for any tissue or cell type in any biological procedure, for diagnosis, prognosis and drug screening.
  • The platform enables fast and reliable screening for epigenetic effects of existing and novel drugs.

 

Our Innovation

  • A microarray slide, printed with histone modifications antibodies at optimal concentrations.
  • Unlimited number of antibodies can be printed.
  • Simple user protocol
  • Fast results with fluorescence microscope or a microarray scanner device

A Proof of Concept:

  • Cy3-labelled histones were incubated on an Ab array slide.
  • Antibody-Antigen binding is clearly observed versus the controls.

Two Alternate end-user protocols possible:

 

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Expected Impact

  • A dramatic simplification of the bench work that should replace or amend any other existing methods of histone modification analysis.
  • Efficient time consuming research, management of laboratories working power and funds.
  • Personalized medicine – the product will allow researchers and clinicians to analyze cell tissues for modified histones making the method a potentially useful tool for prognosis of cancer and neurodegenerative diseases. 

Contact for more information:

Ariela Markel
VP, Business Development, Healthcare
+972-2-6586608
Contact ME:
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